Development of Reverse Transcription Loop-Mediated Isothermal Amplification Assays Targeting SARS-CoV-2
Identifieur interne : 000736 ( 2020/Analysis ); précédent : 000735; suivant : 000737Development of Reverse Transcription Loop-Mediated Isothermal Amplification Assays Targeting SARS-CoV-2
Auteurs : Gun-Soo Park [Corée du Sud] ; Keunbon Ku [Corée du Sud] ; Seung-Hwa Baek [Corée du Sud] ; Seong-Jun Kim [Corée du Sud] ; Seung Il Kim [Corée du Sud] ; Bum-Tae Kim [Corée du Sud] ; Jin-Soo Maeng [Corée du Sud]Source :
- The Journal of Molecular Diagnostics : JMD [ 1525-1578 ] ; 2020.
Abstract
Epidemics of coronavirus disease 2019 (COVID-19) now have >100,000 confirmed cases worldwide. Diagnosis of COVID-19 is currently performed by quantitative RT-PCR methods, but the capacity of quantitative RT-PCR methods is limited by their requirement of high-level facilities and instruments. Herein, reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays to detect genomic RNA of SARS-CoV-2, the causative virus of COVID-19, were developed and evaluated. RT-LAMP assays in this study can detect as low as 100 copies of SARS-CoV-2 RNA. Cross-reactivity of RT-LAMP assays to other human coronaviruses was not observed. A colorimetric detection method was adapted for this RT-LAMP assay so that the tests potentially performed in higher throughput.
Url:
DOI: 10.1016/j.jmoldx.2020.03.006
PubMed: 32276051
PubMed Central: 7144851
Affiliations:
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Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon</wicri:regionArea><wicri:noRegion>Daejeon</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2">Research Group of Food Processing, Korea Food Research Institute, Wanju-gun, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Group of Food Processing, Korea Food Research Institute, Wanju-gun</wicri:regionArea><wicri:noRegion>Wanju-gun</wicri:noRegion></affiliation></author><author><name sortKey="Ku, Keunbon" sort="Ku, Keunbon" uniqKey="Ku K" first="Keunbon" last="Ku">Keunbon Ku</name><affiliation wicri:level="1"><nlm:aff id="aff1">Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du 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Sud</country><wicri:regionArea>Department of Predictive Toxicology, Korea Institute of Toxicology, Daejeon</wicri:regionArea><wicri:noRegion>Daejeon</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Seong Jun" sort="Kim, Seong Jun" uniqKey="Kim S" first="Seong-Jun" last="Kim">Seong-Jun Kim</name><affiliation wicri:level="1"><nlm:aff id="aff1">Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon</wicri:regionArea><wicri:noRegion>Daejeon</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Seung Il" sort="Kim, Seung Il" uniqKey="Kim S" first="Seung Il" last="Kim">Seung Il Kim</name><affiliation wicri:level="1"><nlm:aff id="aff1">Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon</wicri:regionArea><wicri:noRegion>Daejeon</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4">Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Bum Tae" sort="Kim, Bum Tae" uniqKey="Kim B" first="Bum-Tae" last="Kim">Bum-Tae Kim</name><affiliation wicri:level="1"><nlm:aff id="aff1">Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon</wicri:regionArea><wicri:noRegion>Daejeon</wicri:noRegion></affiliation></author><author><name sortKey="Maeng, Jin Soo" sort="Maeng, Jin Soo" uniqKey="Maeng J" first="Jin-Soo" last="Maeng">Jin-Soo Maeng</name><affiliation wicri:level="1"><nlm:aff id="aff1">Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon</wicri:regionArea><wicri:noRegion>Daejeon</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2">Research Group of Food Processing, Korea Food Research Institute, Wanju-gun, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Group of Food Processing, Korea Food Research Institute, Wanju-gun</wicri:regionArea><wicri:noRegion>Wanju-gun</wicri:noRegion></affiliation></author></analytic><series><title level="j">The Journal of Molecular Diagnostics : JMD</title><idno type="ISSN">1525-1578</idno><idno type="eISSN">1943-7811</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Epidemics of coronavirus disease 2019 (COVID-19) now have >100,000 confirmed cases worldwide. Diagnosis of COVID-19 is currently performed by quantitative RT-PCR methods, but the capacity of quantitative RT-PCR methods is limited by their requirement of high-level facilities and instruments. Herein, reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays to detect genomic RNA of SARS-CoV-2, the causative virus of COVID-19, were developed and evaluated. RT-LAMP assays in this study can detect as low as 100 copies of SARS-CoV-2 RNA. Cross-reactivity of RT-LAMP assays to other human coronaviruses was not observed. A colorimetric detection method was adapted for this RT-LAMP assay so that the tests potentially performed in higher throughput.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Zhou, P" uniqKey="Zhou P">P. Zhou</name></author><author><name sortKey="Yang, X L" uniqKey="Yang X">X.L. Yang</name></author><author><name sortKey="Wang, X G" uniqKey="Wang X">X.G. 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Wu</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Park, Gun Soo" sort="Park, Gun Soo" uniqKey="Park G" first="Gun-Soo" last="Park">Gun-Soo Park</name></noRegion><name sortKey="Baek, Seung Hwa" sort="Baek, Seung Hwa" uniqKey="Baek S" first="Seung-Hwa" last="Baek">Seung-Hwa Baek</name><name sortKey="Baek, Seung Hwa" sort="Baek, Seung Hwa" uniqKey="Baek S" first="Seung-Hwa" last="Baek">Seung-Hwa Baek</name><name sortKey="Kim, Bum Tae" sort="Kim, Bum Tae" uniqKey="Kim B" first="Bum-Tae" last="Kim">Bum-Tae Kim</name><name sortKey="Kim, Seong Jun" sort="Kim, Seong Jun" uniqKey="Kim S" first="Seong-Jun" last="Kim">Seong-Jun Kim</name><name sortKey="Kim, Seung Il" sort="Kim, Seung Il" uniqKey="Kim S" first="Seung Il" last="Kim">Seung Il Kim</name><name sortKey="Kim, Seung Il" sort="Kim, Seung Il" uniqKey="Kim S" first="Seung Il" last="Kim">Seung Il Kim</name><name sortKey="Ku, Keunbon" sort="Ku, Keunbon" uniqKey="Ku K" first="Keunbon" last="Ku">Keunbon Ku</name><name sortKey="Maeng, Jin Soo" sort="Maeng, Jin Soo" uniqKey="Maeng J" first="Jin-Soo" last="Maeng">Jin-Soo Maeng</name><name sortKey="Maeng, Jin Soo" sort="Maeng, Jin Soo" uniqKey="Maeng J" first="Jin-Soo" last="Maeng">Jin-Soo Maeng</name><name sortKey="Park, Gun Soo" sort="Park, Gun Soo" uniqKey="Park G" first="Gun-Soo" last="Park">Gun-Soo Park</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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